Effective Construction of DNA Vaccines Against Variable Influenza Genes by Homologous Recombination
نویسندگان
چکیده
منابع مشابه
DNA vaccines against influenza.
Genetic vaccine technology has been considerably developed within the last two decades. This cost effective and promising strategy can be applied for therapy of cancers and for curing allergy, chronic and infectious diseases, such as a seasonal and pandemic influenza. Despite numerous advantages, several limitations of this technology reduce its performance and can retard its commercial exploit...
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Adenoviruses are used extensively to deliver genes into mammalian cells, particularly where there is a requirement for high-level expression of transgene products in cultured cells, or for use as recombinant viral vaccines or in gene therapy. In spite of their usefulness, the construction of adenoviral vectors (AdV) is a cumbersome and lengthy process that is not readily amenable to the generat...
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Recombination of homologous genes is a powerful mechanism for generating sequence diversity, and can be applied to protein analysis and directed evolution. In vitro recombination methods such as DNA shuffling are very flexible and can give hybrid genes with multiple crossovers; they have been used extensively to evolve proteins with improved and novel properties. In vivo recombination in both E...
متن کاملFunctions of homologous DNA recombination
Homologous DNA (or genetic) recombination plays important roles in heredity and evolution. A recent study has shown that a physical linkage established by recombination between two or more homologous DNA regions or molecules is essential for the precise disjunction of homologous chromosomes in meiosis and the recovery of collapsed DNA replication forks. These physical linkages in recombination ...
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Over the past decade, the focus of cloning has shifted from constructing plasmids that express a single gene of interest to creating multigenic constructs that contain entire pathways or even whole genomes. Traditional cloning methods that rely on restriction digestion and ligation are limited by the number and size of fragments that can efficiently be combined. Here, we focus on the use of hom...
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ژورنال
عنوان ژورنال: Virology
سال: 2000
ISSN: 0042-6822
DOI: 10.1006/viro.2000.0199